One major advantage about these libraries is that the lesser use of human immune system make them self-targeting. Wide variety of recombinant antibodies can be generated by random cloning and combination of various immunoglobin genes. This type of antibody library applies less of immune system but more use of creative engineering. So, resulting recombinant antibodies have low antigenic affinity. Therefore, these libraries generate a large variety of recombinant antibody molecules for broad range of antigens.īut one major problem is that lack of immunization results in absence of affinity maturation of the immunoglobin genes. These are large libraries based on isolated B cells from human body. There is no need of immunization for this kind of libraries. Therefore, researchers develop immune libraries with most closely related mammalian species such as mice. This kind of immunization and antibody production by humans is ethically wrong. However, developing immune response in humans is a problem as humans cannot be manipulated like lab animals. This immunization creates high immune response against the antigen and through this researchers achieve affinity maturation of immunoglobin genes. Immunization of mammalian cells such as mice and later B cell are isolation form immune library. These are immune libraries, naïve libraries and phage display libraries. There are three types of libraries that either store immunized B cells or the genes that codes for antibodies. Researchers develop recombinant antibodies in labs by various techniques. These genes also control antibody specificity and affinity for antigen. The expression of certain immunoglobin genes results in antibodies development. Each immunoglobin or antibody molecule is highly specific for the type of antigen to which they bind. ![]() The upper part is variable region and also the antigen binding site while the lower part is constant region and depicts particular class of antibodies. Structure of antibody moleculeĪntibody is a Y-shape molecule, consisting of light and two heavy chains. Initially researchers tried their best for production of humanized antibodies and fragmentation of these Abs and it resulted in production of second and third generation antibodies. The emergence of recombinant DNA technology in 1970’s has led to the production of ‘engineered or recombinant’ antibodies (rAbs). The B cells of adapted immune system produces antibodies or immunoglobin molecules in response to foreign antigens.
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